RNA-seq analysis of chondrocytes treated with periostin

In this project, our objective is to investigate the impact of periostin on global gene expression in chondrocytes. Chondrocytes will be exposed to recombinant periostin, and RNA-seq analysis will be performed to examine the expression of genes both up-regulated and down-regulated in response to periostin treatment.

Expected outcome from the Student's work

Students will acquire knowledge in primary cell culture and the treatment of cells with recombinant proteins.
Students will develop the skills to isolate RNA for RNA-seq analysis.
Students will gain proficiency in basic RNA-seq data handling and analysis.
Students will acquire proficiency in real-time PCR techniques.
This program aims to improve students’ comprehension of fundamental concepts in cell culture and molecular biology.
Depending on the students’ intellectual and practical contribution, the student(s) may become eligible to be a co-author of the work when it is published.

Terms & conditions

Students are required to dedicate a minimum of 4 hours per day to lab work.
Students must complete lab-related EHS trainings.
It is essential that students exhibit self-motivation and possess a solid understanding of the fundamental concepts of basic research within the research lab.
Students are expected to adhere to all procedures established by the program.