Genotyping Analysis Facility

June 15, 2020

The Genotyping Analysis by Microarray provides a comprehensive view of the genome to explore genetic variants such as single nucleotide polymorphisms (SNPs) and large structural changes in DNA among millions of markers, which help in identifying potential causative disease variants for further targeted studies

Researchers at the Center for Biotechnology are using this technology to identify regions of the human genome that contribute to disease susceptibility and phenotypes within the Arabian population, such as diabetes and cardiovascular disease. 

This Microarray-based approach provides trusted performance and supports high-throughput, multiplex processing for genome-wide association studies (GWAS), variant screening, precision medicine research, and other human genotyping projects.

Some of the noteworthy equipment under this facility:

  • The Illumina iScan System is a laser-based, high-resolution imaging tool for genome-wide or targeted genotyping applications, that can rapidly scan and collect large volumes of data from Illumina DNA analysis and RNA analysis high-density BeadChips.

The Genotyping Analysis by Real-time PCR offers sensitivity and specificity for detecting target nucleic acids, making it a useful and powerful technology for research applications.

This fluorescent-based method is useful for understanding disease mechanisms by comparing the expression of different genes in real-time from healthy and non-healthy samples and analyzing particular genetic variants that can lead to major changes associated with diseases.

Some of the noteworthy equipment under this facility:

  • The ViiA™ 7 Real-Time PCR System combines all the qPCR features in a single high-performance instrument, with minimal well-to-well and instrument-to-instrument variation.

 

Other major pieces of equipment at the Center for Biotechnology for DNA processing:

 

  • MagPurix 24 Nucleic Acid Extraction System – This machine is a fully automated system for molecular biology sample preparation from a wide range of biological specimens. It offers the flexibility to process 1-24 samples per run simultaneously, making it ideal for laboratories requiring nucleic acid purification of small to middle sample throughputs

 

  • NanoDrop™ One Spectrophotometer – The Thermo Scientific™ NanoDrop™ One UV-Vis Spectrophotometers is developed for microvolume quantification and analysis of purified nucleic acids and a wide variety of proteins

 

  • Qsonica Sonicator (Q800R2) – Is designed for DNA and Chromatin shearing. DNA can be sheared to specific size ranges making it an ideal system for preparing samples for Next-Generation Sequencing

 

  • Fragment Analyzer – The Agilent Fragment Analyzer system is a fluorescence-based capillary electrophoresis instrument for the accurate sizing, quantification, and qualification of nucleic acids (DNA and RNA).

 

  • Veriti® Thermal Cycler – The Applied Biosystems Veriti® 96-Well Thermal Cycler with the added temperature-control technology is expected to increase the accuracy and efficiency of the polymerase chain reaction (PCR) process, providing precise control over the PCR optimization

 

  • DS-11 Series Spectrophotometer / Fluorometer – DeNovix: The DS-11 FX Series combines fluorescence, microvolume absorbance, and optional cuvette absorbance methods in a single instrument, making it ideal for rapid nucleic acid and protein quantification.